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Evaluation of natural biomaterials made of chitosan for the regeneration of tissues
The use of natural polymers in the production of structures used to stimulate cell development and tissue regeneration is being a promising strategy in tissue engineering.
Objetivos - Metodologia - Resultados - Discussão dos Resultados/Objectives - Methodology - Results - Discussion of Results/Objetivos - Metodología - Resultados - Discusión de los resultados
In this study, chitosan and its derivatives were produced and evaluated alone or associated with Buriti oil and Salmon fish scale regarding their influence on the viability of human keratinocyte cell. Cells from the immortalized keratinocyte line (HaCaT) were seeded at a 5,000/well density, directly in 96 well tissue culture plates (TCPs). After 24 hours, the cells were treated with culture medium (control), 0.13% of chitosan, 0.13% of chitosan conjugated with Salmon fish scales or 0.30% of chitosan conjugated with Buriti oil. Cell viability was assessed based on (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) (MTT) assay after four days cultivation. The results of cell viability showed statistical significance between the cells cultivated in the well-plate (control group) and chitosan tested alone. The mean and standard deviation values obtained from the normalized results of the cell viability (%) of the treated groups compared to the TCPs (used as a control group) were 100±10.1% and the chitosan only was 87.6±9.7% (p<0.01). In contrast, cultivation of both types of conjugated chitosan conjugated were higher than the chitosan alone and similar to the control. The value obtained for chitosan conjugated with Buriti oil was 100.3±6.8% (ns) and 95.9±15.2% (ns) with the fish scales.
Considerações Finais/Final considerations/Consideraciones finales
Therefore, it is possible to affirm that the association of chitosan with Buriti oil and Salmon scales was beneficial for the viability of the cultivated cells, being promising biomaterials for tissue regeneration.
Acknowledgments: MCTIC, FINEP, CNPq, Biomatter P&D of Biomaterials and Stem Cell Research Institute (IPCT).
Palavras-chave/Key words/Palabras clave
natural polymers, biopolymers, biomaterials, regenerative medicine, Salmon fish scales.
GABRIELA MORAES MACHADO, RAFAELA HARTMANN KASPER, NATASHA MAURMANN, CAREN SERRA BAVARESCO, LUCIANO PIGHINELLI, PATRICIA PRANKE